Composite

Part:BBa_K3799010:Design

Designed by: Lakshmi Prakash   Group: iGEM21_IISER_Kolkata   (2021-10-01)


AIP-1 sensor of S.aureus under pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All the sequences were codon optimized for Bacillus subtilis host.


Source

The two component signalling system is present in S.aureus.Pveg promoter is found in Bacillus subtilis

References